Biocontrol Potential of Grapevine Endophytic and Rhizospheric Fungi Against Trunk Pathogens

Grapevine Trunk Diseases (GTDs) are a major challenge to the grape industry worldwide. GTDs responsible for considerable loss of quality, production, and vineyard longevity. Seventy-five percent Chilean vineyards estimated be affected by GTDs. complex diseases caused several fungi species, including members Botryosphaeriaceae family Phaeomoniella chlamydospora , considered some most important causal agents these in Chile. In this study, we isolated 169 endophytic 209 rhizospheric from grapevines grown under organic conventional farming Multiple isolates Chaetomium sp., Cladosporium Clonostachys rosea, Epicoccum nigrum, Purpureocillium lilacinum Trichoderma sp. were evaluated their potential biocontrol activity against Diplodia seriata Neofusicoccum parvum Pa. chlamydospora. Tests antagonism carried out using two dual-culture-plate methods with multiple media types, agar containing grapevine wood extract simulate planta nutrient conditions. Significant pathogen growth inhibition was observed all tested. rosea showed 98.2% pathogens presence extract. We 100% when autoclaved lignified shoots pre-inoculated either C. strains or Overall, results show that promising